6Y2Z

NG domain of human SRP54

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 21 21 21
Unit cell lengths	50.443, 87.668, 170.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.371 - 2.150
R-factor	0.2043
Rwork	0.202
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l3q
RMSD bond length	0.008
RMSD bond angle	0.860
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.371	2.230
High resolution limit [Å]	2.150	2.150
Rpim	0.056
Number of reflections	42073	4140
<I/σ(I)>	10.75
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	na2hpo4 0.8 M K2HPO4 0.8 M HEPES 0.1 M (pH 7.5)