6Y2S
Escherichia coli R318A RnlA endoribonuclease (single alanine mutant of RnlA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-14 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.980126 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.560, 105.560, 156.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.770 - 3.790 |
R-factor | 0.2677 |
Rwork | 0.264 |
R-free | 0.34180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i8o |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.770 | 3.920 |
High resolution limit [Å] | 3.790 | 3.790 |
Rmerge | 1.250 | |
Number of reflections | 10617 | 941 |
<I/σ(I)> | 3.61 | 1.1 |
Completeness [%] | 94.4 | |
Redundancy | 3.87 | |
CC(1/2) | 0.956 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris(2-carboxyethyl) phosphine. Reservoir solution: 0.4 M Potassium sodium tartrate tetrahydrate. |