6Y2N

Crystal structure of ribonucleotide reductase R2 subunit solved by serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	64.320, 64.320, 153.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.300 - 2.400
R-factor	0.1757
Rwork	0.173
R-free	0.22100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	Unpublished
RMSD bond length	0.003
RMSD bond angle	0.492
Data reduction software	CrystFEL (0.7.0)
Data scaling software	CrystFEL (0.7.0)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.300	2.580
High resolution limit [Å]	2.400	2.400
Number of reflections	13245	2336
<I/σ(I)>	6.73
Completeness [%]	98.6
Redundancy	516
CC(1/2)	0.990	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.5	294	16% (w/v) polyethylene glycol 3350, 2% (v/v) tacsimate pH 4.5