6Y2C

Crystal structure of the third KH domain of FUBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.918
Spacegroup name	P 32 2 1
Unit cell lengths	47.409, 47.409, 147.893
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.090 - 2.000
R-factor	0.205
Rwork	0.203
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lij
RMSD bond length	0.015
RMSD bond angle	1.455
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.100	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	13595	999
<I/σ(I)>	8.3
Completeness [%]	98.8
Redundancy	4.5
CC(1/2)	0.995	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG 6000, 10% ethylene glycol, 0.015M Zinkchlorid, 0.1 M MES, pH 6.0