6Y22

RING-DTC domains of Deltex 2, Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97997
Spacegroup name	P 32 2 1
Unit cell lengths	106.418, 106.418, 74.669
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.008 - 2.069
R-factor	0.1807
Rwork	0.179
R-free	0.20830
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.836
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	PHENIX (1.10_2142)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.160	2.120
High resolution limit [Å]	2.069	2.069
Rmerge	0.076	0.748
Rpim	0.037	0.360
Number of reflections	29929	2130
<I/σ(I)>	16.5	3.3
Completeness [%]	99.7	97.3
Redundancy	10	10
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	292	Morpheus condition 60: 0.12 M ethylene glycols (0.3M Diethylene glycol; 0.3M Triethylene glycol; 0.3M Tetraethylene glycol; 0.3M Pentaethylene glycol), 0.1 M Buffer system 3 (1 M Tris-Bicine pH 8.5), 37.5% Precipitation mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)