6Y0Q
Alpha-ketoglutarate-dependent dioxygenase AlkB in complex with Fe, AKG and methylated DNA under anaerobic environment using FT-SSX methods
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.399, 39.078, 40.840 |
| Unit cell angles | 78.99, 78.04, 66.88 |
Refinement procedure
| Resolution | 39.660 - 1.750 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jht |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.715 |
| Data reduction software | DIALS (1.8) |
| Data scaling software | DIALS (1.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 20346 | 1028 |
| <I/σ(I)> | 37.21 | 2.81 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 57.57 | 10.6 |
| CC(1/2) | 0.977 | 0.255 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | 25% w/v PEG 3350, 5% v/v glycerol and 200 mM sodium formate |
