6XZN

Arabidopsis UV-B photoreceptor UVR8 mutant G101S W285A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	50.541, 99.123, 132.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.400 - 1.750
R-factor	0.1585
Rwork	0.155
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d9s
RMSD bond length	0.010
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.813
High resolution limit [Å]	1.750	1.751
Number of reflections	67187	6556
<I/σ(I)>	21.71
Completeness [%]	98.7
Redundancy	13.4
CC(1/2)	1.000	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2M Sodium nitrate 0.1M Bis Tris propane pH 8.5 20% w/v PEG 3350. 20% Glycerol cryoprotectant.