6XZL

Arabidopsis UV-B photoreceptor UVR8 mutant D96N D107N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	97.614, 50.980, 70.488
Unit cell angles	90.00, 104.95, 90.00

Refinement procedure

Resolution	47.160 - 1.390
R-factor	0.1458
Rwork	0.144
R-free	0.17930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d9s
RMSD bond length	0.010
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	1.440
High resolution limit [Å]	1.390	1.390
Number of reflections	67424	6711
<I/σ(I)>	8.97
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.996	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Methylated protein. 0.1M TRIS 8.5, 0.1M NaCl, 30% PEG4000, 1.8mg/mL, 2:1, prot:buffer. 25% glycerol cryoprotectant