6XZ6
Structure of the trypanosome brucei factor H receptor bound to domain D5 of bovine factor H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.505, 66.056, 71.018 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 27.770 - 2.700 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e4o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.770 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 20315 | 1049 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.978 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M ammonium acetate, 17 % (w/v) polyethylene glycol 10,000, 0.1 M Bis-(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, pH 5.5 |
