6XY6

Structural insight into sheep-pox virus mediated inhibition of apoptosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	100.279, 78.560, 107.766
Unit cell angles	90.00, 110.97, 90.00

Refinement procedure

Resolution	46.819 - 2.914
R-factor	0.241186273945
Rwork	0.239
R-free	0.28834
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SPPV_Hrk
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	XDS (20180427)
Data scaling software	Aimless (7.0)
Phasing software	PHASER (1.11.1-2575-000)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.819	3.060
High resolution limit [Å]	2.910	2.910
Rmerge	0.150	1.244
Number of reflections	34323	4465
<I/σ(I)>	5.2
Completeness [%]	99.6
Redundancy	3.5
CC(1/2)	0.990	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	1.0 M Lithium chloride, 0.1 M Citrate pH 4.0, 20% w/vPEG 6000