6XXS
Crystal structure of an NCoR1BBD2-BCL6BTB chimera in complex with the NcoR1 BBD1 corepressor peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 165.380, 165.380, 244.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 82.690 - 3.250 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r28 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.575 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.050 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rpim | 0.170 | 0.818 |
| Number of reflections | 31807 | 4532 |
| <I/σ(I)> | 5.5 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | |
| CC(1/2) | 0.958 | 0.154 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2M Sodium chloride 5%(w/v) PEG 4000 0.1M Tris base/ Hydrochloric acid pH 8.5 |
