6XXG
Structure of truncated 1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Deinococcus radiodurans
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.321, 87.367, 181.865 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.549 - 2.099 |
Rwork | 0.191 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o1x |
RMSD bond length | 0.006 |
RMSD bond angle | 1.402 |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190315) |
Data scaling software | XDS (VERSION Mar 15, 2019 BUILT=20190315) |
Phasing software | MOLREP (Vers 11.7.02; 29.05.2019) |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.549 | 2.175 |
High resolution limit [Å] | 2.099 | 2.100 |
Number of reflections | 67987 | 6677 |
<I/σ(I)> | 5.87 | |
Completeness [%] | 99.1 | 99.48 |
Redundancy | 2 | |
CC(1/2) | 0.913 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Calcium acetate hydrate, 0.1 M Tris, 20 % PEG 4000 |