6XWY

Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	92.020, 96.861, 232.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.430 - 1.750
R-factor	0.2048
Rwork	0.203
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0m
RMSD bond length	0.007
RMSD bond angle	1.139
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	1.812
High resolution limit [Å]	1.750	1.750
Number of reflections	202826	10267
<I/σ(I)>	18.37
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	50 mM Tris pH 8.0 0.2 M Ammonium Sulfate 26 % PEG 8000