6XWX

Crystal structure of the Tof1-Csm3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	C 1 2 1
Unit cell lengths	264.472, 82.034, 161.165
Unit cell angles	90.00, 124.85, 90.00

Refinement procedure

Resolution	45.730 - 3.100
R-factor	0.238
Rwork	0.237
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mqi
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.740	3.524
High resolution limit [Å]	3.090	3.090
Rpim	0.078	0.737
Number of reflections	27164	1359
<I/σ(I)>	7.1	1.2
Completeness [%]	89.8
Redundancy	7
CC(1/2)	0.996	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium Acetate, 0.1 M Tris-HCl pH 7.5-8.5, 4-8% PEG 20000