6XWV

Crystal structure of drosophila melanogaster CENP-C bound to CAL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	86.273, 86.444, 88.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.080 - 2.270
R-factor	0.2389
Rwork	0.237
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	dmCENP-C cupin
RMSD bond length	0.006
RMSD bond angle	1.187
Data reduction software	XDS (1.17.1_3660)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	2.350
High resolution limit [Å]	2.270	2.270
Rmerge	0.076	0.660
Number of reflections	30957	2932
<I/σ(I)>	21.2
Completeness [%]	98.4
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Calcium acetate, 0.1M Na Cacodylate, pH 6.5, 18% PEG 8000