6XWU

Crystal structure of drosophila melanogaster CENP-C cumin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	57.195, 57.195, 92.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.600 - 1.820
R-factor	0.1976
Rwork	0.194
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpv
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	1.885
High resolution limit [Å]	1.820	1.820
Rmerge	0.055	0.055
Number of reflections	14427	1392
<I/σ(I)>	34.13
Completeness [%]	99.7
Redundancy	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50mM MES pH6.0, 45% PEG 400