6XWS
Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A/H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 199.700, 199.700, 76.754 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 172.950 - 4.360 |
| R-factor | 0.3072 |
| Rwork | 0.306 |
| R-free | 0.32290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pyo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 172.950 | 5.186 |
| High resolution limit [Å] | 4.360 | 4.360 |
| Rmerge | 0.129 | 0.950 |
| Number of reflections | 2322 | 194 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 89.0 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.5 and 30% PEG 4000 |
