6XU4
Crystal structure of the genetically-encoded FGCaMP calcium indicator in its calcium-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 163.561, 163.561, 143.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.940 - 3.180 |
| R-factor | 0.2825 |
| Rwork | 0.279 |
| R-free | 0.34830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 89.940 | 89.940 | 3.340 |
| High resolution limit [Å] | 3.180 | 10.550 | 3.180 |
| Rmerge | 0.098 | 0.039 | 0.876 |
| Rmeas | 0.106 | 0.044 | 0.947 |
| Rpim | 0.040 | 0.020 | 0.353 |
| Total number of observations | 6250 | 30518 | |
| Number of reflections | 33268 | 1048 | 4353 |
| <I/σ(I)> | 13.6 | 34.9 | 2.4 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 7 | 6 | 7 |
| CC(1/2) | 0.985 | 0.976 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.5 M Ammonium sulfate; 10%DMSO |
