6XSN

Crystal structure of NHP VD20.5A4 Fab in complex with 16055 V1V2 1FD6 scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 2 2
Unit cell lengths	106.565, 106.565, 128.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 2.870
R-factor	0.2231
Rwork	0.221
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u3z
RMSD bond length	0.003
RMSD bond angle	0.765
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.600	2.920
High resolution limit [Å]	2.870	2.870
Number of reflections	17667	17702
<I/σ(I)>	10.88
Completeness [%]	100.0	99.9
Redundancy	12.7
CC(1/2)	1.000	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Na Acet 5.5 pH 10%w/v PEG 8K 10%w/v PEG 1K 0.2M KSCN