6XSF
Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-09-13 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.220, 60.460, 38.240 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 24.880 - 1.600 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.570 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.170 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.015 | 0.102 |
| Number of reflections | 18805 | 1885 |
| <I/σ(I)> | 58.61 | 8.82 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 35% MPD, 25 mM potassium phosphate, calcium chloride, THP |
