6XS4
Crystal structure of glycyl radical enzyme ECL_02896 from Enterobacter cloacae subsp. cloacae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 283.101, 60.974, 118.951 |
Unit cell angles | 90.00, 107.65, 90.00 |
Refinement procedure
Resolution | 39.720 - 2.330 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.20330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mtj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.724 | 2.380 |
High resolution limit [Å] | 2.330 | 2.340 |
Rpim | 0.045 | 0.192 |
Number of reflections | 78987 | 3022 |
<I/σ(I)> | 9.25 | |
Completeness [%] | 95.4 | |
Redundancy | 3.5 | |
CC(1/2) | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 15% PEG3350, 0.1M Succinic Acid pH 7 |