6XRX

Crystal structure of the mosquito protein AZ1 as an MBP fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	113.013, 113.013, 83.442
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.820 - 1.950
R-factor	0.1778
Rwork	0.176
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t05 4jrb
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmeas	0.056	0.861
Rpim	0.022	0.337
Number of reflections	44356	2211
<I/σ(I)>	10.9	2.26
Completeness [%]	100.0	100
Redundancy	6.6	6.5
CC(1/2)		0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG 1000, 50mM MES, 100mM NaCl, 200mM MgCl2