6XQK
Crystal structure of the D/D domain of PKA from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.824, 52.134, 68.832 |
| Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
| Resolution | 64.800 - 2.560 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27000 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.800 | 2.690 |
| High resolution limit [Å] | 2.557 | 2.560 |
| Rmerge | 0.125 | 0.440 |
| Number of reflections | 12426 | 1777 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% w/v PEG4000, 0.1 M Tris, pH 8.5, 0.2 M magnesium chloride |
