6XQD

Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	208.330, 447.600, 612.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.460 - 2.800
R-factor	0.2272
Rwork	0.225
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.006
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.460	75.460	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.214	0.031	1.662
Rmeas	0.247	0.036	1.912
Total number of observations	5485119
Number of reflections	1364434	15561	101514
<I/σ(I)>	6.04	26.8	0.84
Completeness [%]	98.4	94.6	99.4
Redundancy	4.02	4.045	4.056
CC(1/2)	0.989	0.998	0.131

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME