6XQ6
Receptor for Advanced Glycation End Products VC1 domain in complex with Fragment 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 62 |
| Unit cell lengths | 101.376, 101.376, 104.025 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.440 - 1.900 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lp4 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.374 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.076 | 0.286 |
| Rpim | 0.026 | 0.100 |
| Number of reflections | 94132 | 9443 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.1 | 7.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5 M NaOAc (pH 7.5) |
