6XQ4

Human antibody S8V2-47 in complex with the influenza hemagglutinin head domain of A/Beijing/262/1995(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	96.090, 66.470, 135.180
Unit cell angles	90.00, 101.96, 90.00

Refinement procedure

Resolution	49.475 - 3.347
R-factor	0.2401
Rwork	0.238
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 6Q0E 4tsb 6b5l & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.475	3.467
High resolution limit [Å]	3.347	3.347
Rmerge	0.052	0.303
Rmeas	0.073	0.429
Rpim	0.052	0.303
Number of reflections	23648	2357
<I/σ(I)>	9.38	2.1
Completeness [%]	96.6	97.92
Redundancy	2	2
CC(1/2)	0.997	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	0.1 M sodium chloride, 12% w/v PEG4000 , 0.1 M trisodium citrate, pH 5.6