6XQ2

Human antibody S8V2-37 in complex with the influenza hemagglutinin head domain of A/Texas/50/2012(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	C 1 2 1
Unit cell lengths	294.800, 42.380, 155.760
Unit cell angles	90.00, 111.50, 90.00

Refinement procedure

Resolution	49.059 - 3.002
R-factor	0.2494
Rwork	0.247
R-free	0.29020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5W08 6foe 4m6o & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.059	3.110
High resolution limit [Å]	3.002	3.002
Rmerge	0.153	0.999
Rmeas	0.175	1.140
Rpim	0.083	0.538
Number of reflections	36359	3560
<I/σ(I)>	9.71	1.28
Completeness [%]	98.3	98.09
Redundancy	4.1	4.2
CC(1/2)	0.994	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	0.1 M magnesium sulfate, 20% w/v PEG4000, sodium acetate, pH 5.0