6XQ0

Human antibody S8V2-18 in complex with the influenza hemagglutinin head domain of A/California/7/2009(NYMC-X181)(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	160.200, 55.260, 168.000
Unit cell angles	90.00, 95.02, 90.00

Refinement procedure

Resolution	44.880 - 2.300
R-factor	0.2243
Rwork	0.222
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3UBE 4u6v 4lrn & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.880	2.383
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	0.674
Rmeas	0.134	0.826
Rpim	0.077	0.471
Number of reflections	62605	6233
<I/σ(I)>	8.03	1.34
Completeness [%]	95.2	96.14
Redundancy	2.8	2.8
CC(1/2)	0.991	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	25% w/v PEG3350, 0.1 M Tris, pH 8.5