6XND

Avidin-Biotin-Phenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	77
Detector technology	CCD
Collection date	2020-02-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	46.526, 79.254, 74.683
Unit cell angles	90.00, 105.46, 90.00

Refinement procedure

Resolution	44.840 - 1.580
R-factor	0.2094
Rwork	0.209
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2avi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.843	1.640
High resolution limit [Å]	1.580	1.580
Number of reflections	71463	6991
<I/σ(I)>	10.23
Completeness [%]	99.9
Redundancy	6.4
CC(1/2)	0.999	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	300	0.1 M Tris, pH 7.0, and 25% (w/v) PEG 1500