6XND
Avidin-Biotin-Phenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2020-02-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.526, 79.254, 74.683 |
| Unit cell angles | 90.00, 105.46, 90.00 |
Refinement procedure
| Resolution | 44.843 - 1.580 |
| R-factor | 0.2351 |
| Rwork | 0.234 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2avi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.843 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 71463 | 6991 |
| <I/σ(I)> | 10.23 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.999 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 0.1 M Tris, pH 7.0, and 25% (w/v) PEG 1500 |
