6XMD

SM Protein Vps45 in Complex with Qa SNARE Tlg2 (1-310)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97935
Spacegroup name	P 21 2 21
Unit cell lengths	58.380, 89.434, 209.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.510 - 3.900
R-factor	0.1932
Rwork	0.191
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xjl
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.510	29.510	4.340
High resolution limit [Å]	3.880	8.670	3.880
Rmerge	0.246	0.088	2.149
Rmeas	0.256	0.092	2.234
Rpim	0.071	0.027	0.603
Total number of observations	138267	12417	38856
Number of reflections	10653	1040	2891
<I/σ(I)>	6.9	27.5	1.3
Completeness [%]	99.3	97.8	98.1
Redundancy	13	11.9	13.4
CC(1/2)	0.993	0.990	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4 mg/ml protein in well buffer consisting of 0.125 M potassium citrate, 15 % (w/v) PEG 3350, 15% (v/v) glycerol, and 1 mM DTT