6XM1

SM Protein Vps45 in Complex with Qa SNARE Tlg2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	58.727, 180.057, 202.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.680 - 2.800
R-factor	0.1972
Rwork	0.194
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xjl
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.680	29.680	2.890
High resolution limit [Å]	2.800	11.540	2.800
Rmerge	0.138	0.030	1.880
Rmeas	0.144	0.032	1.961
Rpim	0.039	0.009	0.549
Total number of observations		10199	55507
Number of reflections	53946	841	4521
<I/σ(I)>	13.6	47.9	1.4
Completeness [%]	99.8	94.6	98.9
Redundancy	13.4	12.1	12.3
CC(1/2)	0.999	1.000	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2.5 mg/ml protein in well buffer, consisting of 0.1 M HEPES pH 8.0, 0.1 M NaCl, 11% (v/v) 2-propanol, and 5 mM TCEP