6XLZ

Structure of NHP D11A.F2 Fab in complex with 16055 V2b peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	P 61 2 2
Unit cell lengths	130.393, 130.393, 170.898
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	94.210 - 2.800
R-factor	0.2183
Rwork	0.216
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rfo
RMSD bond length	0.010
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.220	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.083
Number of reflections	21787	3087
<I/σ(I)>	11.9
Completeness [%]	99.8	98.9
Redundancy	22.2	22.2
CC(1/2)	0.720	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris pH 8.0 and 1.32 M K/Na Tartrate at 8.5 mg/ml. Cryo protection: 15% 2R3R Butanediol