6XLX
Crystal structure of cancer-associated G301D mutant of U2AF65 bound to AdML splice site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.492, 61.875, 77.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.710 - 1.700 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ev3 |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.17.1) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.710 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.063 | 0.570 |
| Rpim | 0.028 | 0.265 |
| Number of reflections | 44173 | 1208 |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 99.7 | 97.1 |
| Redundancy | 5.8 | 5.2 |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25% PEG 3350, 0.24 M Na malonate pH 7, 5% sucrose, Lauryldimethylamine oxide |
