6XLP
Structure of the essential inner membrane lipopolysaccharide-PbgA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.272, 56.328, 97.359 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 24.240 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i5h |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_2747) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.242 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.379 |
| Number of reflections | 53215 | 3926 |
| <I/σ(I)> | 15.5 | 1.7 |
| Completeness [%] | 88.0 | 62.1 |
| Redundancy | 2.6 | 1.9 |
| CC(1/2) | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 277 | LCP was formulated from monoolein (Sigma): phosphatidylethanolamine (E. coli PE, Avanti Polar Lipids) 99.5:0.5% m/m mixture at 40% hydration; crystallization solution contained 0.1 M Tris pH 8.0, 0.2 M ammonium sulfate, 40% PEG200 |
