6XKN
Class III PreQ1 riboswitch mutant A52G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6279 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 80.137, 80.137, 281.457 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.710 - 2.730 |
| R-factor | 0.2458 |
| Rwork | 0.244 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4rzd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | HKL-2000 (v708c) |
| Data scaling software | HKL-2000 (v708c) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.710 | 2.830 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rpim | 0.035 | 0.357 |
| Number of reflections | 15078 | 1473 |
| <I/σ(I)> | 20 | 1.7 |
| Completeness [%] | 97.3 | 98.8 |
| Redundancy | 8.4 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 85% Tacsimate pH 7.0, 10 mM magnesium acetate, 6 mM cobalt haxammine, 1 mM spermine |
