6XKB
Crystal structure of SR-related and CTD-associated factor 4(SCAF4-CID)with peptide S2,S5p-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.718, 46.923, 139.250 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 44.500 - 1.600 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.058 | 0.034 | 0.756 |
| Rmeas | 0.064 | 0.037 | 0.829 |
| Rpim | 0.025 | 0.015 | 0.334 |
| Total number of observations | 715061 | ||
| Number of reflections | 114559 | 5989 | 5678 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 94.5 | 94.7 | 94.8 |
| Redundancy | 6.2 | 6 | 5.8 |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 25% PEG 3350, 0.2 M NH4OAc, 0.1 M HEPES pH 7.5 |
