6XJW
Crystal structure of a self-alkylating ribozyme - alkylated form without biotin moiety
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.916, 99.639, 90.544 |
| Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
| Resolution | 89.850 - 1.918 |
| R-factor | 0.1884 |
| Rwork | 0.188 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mwn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.640 | 1.950 |
| High resolution limit [Å] | 1.918 | 1.920 |
| Rmerge | 0.086 | 1.901 |
| Rmeas | 0.101 | 2.239 |
| Rpim | 0.054 | 1.175 |
| Number of reflections | 111123 | 5188 |
| <I/σ(I)> | 8.7 | 0.6 |
| Completeness [%] | 98.3 | 92.8 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.998 | 0.247 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 0.1 M ammonium fluoride, 10% PEG3350, pH 6.2 |
