6XJQ
Crystal structure of a self-alkylating ribozyme - alkylated form with biotinylated epoxide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.962, 99.832, 90.636 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 83.300 - 1.708 |
| R-factor | 0.1894 |
| Rwork | 0.189 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mwn |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.300 | 1.740 |
| High resolution limit [Å] | 1.708 | 1.710 |
| Rmerge | 0.056 | 1.848 |
| Rmeas | 0.066 | 2.187 |
| Rpim | 0.035 | 1.160 |
| Number of reflections | 159200 | 7389 |
| <I/σ(I)> | 11.2 | 0.6 |
| Completeness [%] | 99.3 | 93.3 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.235 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 0.1 M ammonium fluoride, 10% PEG3350, pH 6.2 |
