6XJN

Human atlastin-1 (residues 1-439) bound to GDP-Mg2+ exhibits an ordered amino terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9767
Spacegroup name	I 2 2 2
Unit cell lengths	54.170, 138.860, 202.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.860 - 2.200
R-factor	0.2021
Rwork	0.201
R-free	0.23690
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.898
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge		0.735
Rmeas	0.078
Number of reflections	39278	5640
<I/σ(I)>	16.2
Completeness [%]	100.0
Redundancy	8.9
CC(1/2)	0.998	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	3.5 M sodium formate pH 7, crystals transferred to cryo solution supplemented with 25% glycerol