6XI3
Crystal structure of tetra-tandem repeat in extending region of large adhesion protein
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-18 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.7712 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.833, 45.631, 58.600 |
| Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.000 |
| Rwork | 0.179 |
| R-free | 0.23080 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.642 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 0.471 |
| Rmeas | 0.107 | 0.493 |
| Rpim | 0.028 | 0.144 |
| Number of reflections | 35779 | 2310 |
| <I/σ(I)> | 19.6 | 4.2 |
| Completeness [%] | 99.0 | 87.6 |
| Redundancy | 14.1 | 11.4 |
| CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 296 | PEG 6000, sodium acetate, sodium chloride |
