6XHX

Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with erythromycin and protein Y (YfiA) at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.510, 448.860, 619.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.430 - 2.550
R-factor	0.2241
Rwork	0.222
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.430	224.430	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.258	0.076	2.129
Rmeas	0.272	0.081	2.278
Total number of observations	18783803
Number of reflections	1869146	21939	137715
<I/σ(I)>	7.44	24.24	0.86
Completeness [%]	99.9	99.4	100
Redundancy	10.049	10.38	7.885
CC(1/2)	0.993	0.994	0.163

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME