6XHV

Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	207.740, 444.070, 613.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	208.790 - 2.400
R-factor	0.2395
Rwork	0.237
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	208.790	208.790	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.285	0.071	3.345
Rmeas	0.295	0.074	3.493
Total number of observations	32313767
Number of reflections	2175692	25535	160010
<I/σ(I)>	8.98	32.13	0.8
Completeness [%]	99.9	99.6	99.7
Redundancy	14.852	17.086	11.956
CC(1/2)	0.992	0.993	0.111

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME