6XH1
Co-crystal structure of HIV-1 TAR RNA in complex with lab-evolved RRM TBP6.7 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.226, 40.226, 293.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.850 - 2.601 |
R-factor | 0.2185 |
Rwork | 0.216 |
R-free | 0.25490 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Mar 15, 2019) |
Data scaling software | Aimless (0.74) |
Phasing software | PHENIX (1.15.2_3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.850 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rpim | 0.065 | 0.550 |
Number of reflections | 7660 | 944 |
<I/σ(I)> | 7.7 | 2.4 |
Completeness [%] | 96.5 | 97.7 |
Redundancy | 7.9 | 7.9 |
CC(1/2) | 0.986 | 0.403 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293.15 | 0.2 M Ammonium Nitrate, 20% (w/v) PEG-3350 |