6XGZ

Crystal structure of E. coli MlaFB ABC transport subunits in the monomeric state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1158
Spacegroup name	P 21 21 21
Unit cell lengths	78.270, 136.080, 261.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.870 - 2.600
R-factor	0.2033
Rwork	0.203
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ouk 3f43
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	86874	8606
<I/σ(I)>	18.68
Completeness [%]	92.1
Redundancy	13.1
CC(1/2)	1.000	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291.15	0.7 M NH4H2PO4, 0.07 M Na3 Cit pH5.6, 30 %v/v Glycerol