6XGP
YSD1_17 major capsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.396, 91.994, 89.032 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 30.830 - 2.600 |
| R-factor | 0.2211 |
| Rwork | 0.219 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bqw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS (20151015) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 | 0.734 |
| Rmeas | 0.149 | 1.038 |
| Rpim | 0.106 | 0.734 |
| Number of reflections | 26350 | 2620 |
| <I/σ(I)> | 8 | 1.4 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.983 | 0.438 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 4 % PEG3350, 20 % glycerol, 0.2 M NaBr and 0.1 M MOPS-HEPES buffer (pH 7.5) |
