6XFU
PmtCD peptide exporter basket domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 40.462, 45.375, 73.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.460 - 1.400 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u2d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.871 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.370 | 45.370 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.098 | 0.069 | 0.653 |
| Rmeas | 0.103 | 0.073 | 0.717 |
| Rpim | 0.029 | 0.022 | 0.287 |
| Total number of observations | 314139 | 2126 | 6889 |
| Number of reflections | 27091 | 213 | 1152 |
| <I/σ(I)> | 14.7 | 27.7 | 2.1 |
| Completeness [%] | 98.5 | 99.7 | 85.4 |
| Redundancy | 11.6 | 10 | 6 |
| CC(1/2) | 0.998 | 0.998 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 0.2 M sodium thiocyanate and 20% Poly Ethylene Glycol (PEG) 3350 |
