6XFK

Crystal structure of the type III secretion system pilotin-secretin complex InvH-InvG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 65 2 2
Unit cell lengths	36.313, 36.313, 221.602
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.930 - 1.850
R-factor	0.2189
Rwork	0.217
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xfj
RMSD bond length	0.014
RMSD bond angle	1.766
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.930	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	8226	788
<I/σ(I)>	16.8
Completeness [%]	99.5
Redundancy	16.5
CC(1/2)	1.000	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	0.5 M lithium sulfate, 8.3% PEG 6000, 100 mM sodium acetate