6XF2

Nesprin-1G (aa2070-2200)-FHOD1(aa1-339) complex, H. sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	CCD
Collection date	2018-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9870
Spacegroup name	C 1 2 1
Unit cell lengths	209.367, 142.051, 58.047
Unit cell angles	90.00, 106.31, 90.00

Refinement procedure

Resolution	60.580 - 7.110
R-factor	0.3457
Rwork	0.340
R-free	0.39720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xf1
RMSD bond length	0.009
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.580	7.350
High resolution limit [Å]	7.100	7.100
Rpim	0.063
Number of reflections	2378	232
<I/σ(I)>	15.6
Completeness [%]	98.2
Redundancy	6
CC(1/2)	0.980	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M HEPES/NaOH pH 7.0, 0.2 M sodium thiocyanate, 40% 5/4 pentaerythritol propoxylate