6XC9

Immune receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95370
Spacegroup name	P 1 21 1
Unit cell lengths	65.993, 114.790, 153.926
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	46.010 - 2.400
R-factor	0.1863
Rwork	0.184
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z7u
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.010	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.059	0.444
Rmeas		0.628
Rpim	0.059	0.444
Number of reflections	89707	8924
<I/σ(I)>	8.82	2.13
Completeness [%]	99.9	99.98
Redundancy	2
CC(1/2)	0.996	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	13% PEG 8000, 0.2 M Potassium dihydrogen phosphate