6XC9
Immune receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.993, 114.790, 153.926 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 46.010 - 2.400 |
| R-factor | 0.1863 |
| Rwork | 0.184 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z7u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.622 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 | 0.444 |
| Rmeas | 0.628 | |
| Rpim | 0.059 | 0.444 |
| Number of reflections | 89707 | 8924 |
| <I/σ(I)> | 8.82 | 2.13 |
| Completeness [%] | 99.9 | 99.98 |
| Redundancy | 2 | |
| CC(1/2) | 0.996 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 13% PEG 8000, 0.2 M Potassium dihydrogen phosphate |
