6XBO
X-ray crystal structure of the mouse CMP-Sialic acid transporter in complex with 5-methyl CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03319 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.260, 49.400, 49.830 |
Unit cell angles | 90.00, 107.62, 90.00 |
Refinement procedure
Resolution | 46.149 - 1.800 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6oh2 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.130 | |
Rpim | 0.060 | |
Number of reflections | 31213 | 2821 |
<I/σ(I)> | 7 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.210 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293.15 | 26.7-30% PEG 300, 0.1 M MES pH 6.5, and 0.1 M NaCl |